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| AAS | FTIR | LUMINESCENT AND ABSORPTION | HPLC | CE | MICROWAVE SAMPLE DIGESTION |



Instruments developed at Lumex implement most advanced technologies and analysis techniques achieved in analytical instrumentation engineering.

Types of analysis/ Description Instruments
Atomic Absorption Spectrometry (AAS)

The advantages of the well-known atomic absorption (AA) spectrometry are utilized to the full extent and the technique itself is brought to perfection in the MGA-915 Atomic Absorption Spectrometer and RA-915+ Mercury Analyzer to make them state-of-the art, convenient, accurate, and extremely sensitive instruments due to the use of the Zeeman atomic absorption spectrometry using high frequency modulation of light polarization (ZAAS - HFM).

The high sensitivity and selectivity of the instruments allow the requirements for the sample preparation and its weight to be made much more lenient. The polarization modulation device embodies novel proprietary design features and components.

IR SPECTROMETRY

In modern spectroscopic instruments, the IR absorption, transmission, or scattering spectra are detected by scanning the phase shift between two split parts of a light beam, which is the essence of the Fourier-transform spectrometry. 

This technique provides a significant gain in photometric accuracy and frequency precision. The InfraLUM FT-02 mid-IR FT and InfraLUM FT-10 near-IR FT spectrometers, featuring a state-of-the-art robust vibration- and misalignment-proof design, allow a user to conveniently perform a variety of analytical functions, such as identification of an unknown substance, qualitative and quantitative analysis of solutions, mixed substances, or of complex natural objects, detection of impurities in atmospheric air and gases, and a functional structural analysis. Using a correlation analysis, from the IR spectrum of a sample an analyst can also determine its physical and chemical properties or biological characteristics, such as sprouting ability of seeds, caloric content of food products, grain size, density, etc. To facilitate performing the analyses, the instruments are furnished with a variety of accessories, dedicated SpectraLUM control, data acquisition and processing software package, and calibration and reference databases. The capability of fast identification and detection of many constituents of a complex mixture in a single run brings the performance of these instruments on par with a whole chemical laboratory.

In modern spectroscopic instruments, the IR absorption, transmission, or scattering spectra are detected by scanning the phase shift between two split parts of a light beam, which is the essence of the Fourier-transform spectrometry.

This technique provides a significant gain in photometric accuracy and frequency precision. The InfraLUM FT-02 mid-IR FT, and InfraLUM FT-10 and InfraLUM WholeGrain near-IR FT spectrometers, featuring a state-of-the-art robust vibration- and misalignment-proof design, allow a user to conveniently perform a variety of analytical functions, such as identification of an unknown substance, qualitative and quantitative analysis of solutions, mixed substances, or of complex natural objects, detection of impurities in atmospheric air and gases, and a functional structural analysis. Using a correlation analysis, from the IR spectrum of a sample an analyst can also determine its physical and chemical properties or biological characteristics, such as such as protein, fats, moisture, sugar, cellulose, starch, fibres, etc. To facilitate performing the analyses, the instruments are furnished with a variety of accessories, dedicated SpectraLUM control, data acquisition and processing software package, and calibration and reference databases. The capability of fast identification and detection of many constituents of a complex mixture in a single run brings the performance of these instruments on par with a whole chemical laboratory.


LUMINESCENT AND ABSORPTION PHOTOMETRIC ANALYSIS

For many years the photometric analysis techniques were dominating in most of ecological, sanitary, technological, and biochemical laboratories. However, as the requirements for the ultimate tolerable concentrations (UTC) have become more stringent, the luminescence techniques are being widely used for assaying many of the substances, especially for low detection concentration, and are preferable to the absorption photometric techniques due to the following advantages: 

  • the high selectivity of the luminescence technique allows the number of the sample preparation stages to be reduced and the reliability of the assay to be enhanced (Zn, Cu, Al); 

  • the high sensitivity of the technique allows operation with small-volume samples, thereby speeding up sample processing during each stage of the sample preparation, and reducing the consumption of reagents (surfactants, formaldehyde, Cd, Sn, Cr, Al, Cu); 

  • it is possible to detect the components which are undetectable by the photometric technique because of their low UTC (phenols, As, B, Be, Se, U, B-vitamins) or need the use of a special-purpose IR-absorption photometer (petroleum hydrocarbons).

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)

Liquid chromatography is a well-established highly selective technique for analysis of complex mixtures. When using the HPLC technique for analysis of compounds with the rated low ultimate tolerable concentrations (UTC), such as biogenic amines, polyaromatic hydrocarbons, hormones, and toxins, the sensitivity is of primary importance, because the preparation of proper samples is a laborious procedure. The use of a fluorometric detector, in particular, a FLUORAT® analyzer, provides the high sensitivity to the above substances and thus substantially improves the HPLC performance. Along with some other accessories, the PYTHON pump, developed and manufactured by Lumex, being combined with a FLUORAT® analyzer, makes up a chromatographic system.

CAPILLARY ELECTROPHORESIS (CE)

Capillary electrophoresis (CE, or, originally, high performance capillary electrophoresis) is a relatively new technique which relies on a separation and subsequent quantification of the components of the complex mixtures. The separation is based on the differential migration of the components inside the quartz capillary under the influence of the applied electric field. Lumex has designed and put on the market Capel CE series of instruments which feature all advantages typical for capillary electrophoresis:

  • Since migration of the analytes occurs due to electrically-driven forces, no high pressure precision pumps are needed
  • Very low reagents and samples consumption
  • Very low analysis cost
  • Very fast analysis time
  • The absence of any solid material in the capillary excludes the problems of its aging, chemical or physical destruction or unspecific binding with sample constituents
  • Separation efficiency can be extremely high, up to 1.000.000 theoretical plates

Scope of application: Since CE ensures very high separation efficiency, it can be used for the separation of numerous compounds with very similar structure. It is widely used in pharmacology and pharmacokinetics for the analysis of drugs (including drugs of abuse) in raw materials and in biological fluids, in environmental studies for the analysis of pollutants, inorganic cations and anions, in chemical and food industry for technological and quality control and in various fields of scientific research (analysis of proteins, peptides, oligonucleotides, viruses, etc.).

MORE INFO ABOUT THE METHOD>>

LASER LIGHT SCATTERING

The laser light scattering technique is used for analysis of
microparticles in suspensions and emulsions by measuring the angular diagram of the intensity of the light scattering function. The light sacttering function of a beam passed through a dispersive system depends on the size distribution function, optical characteristics, and the number of particles. 

The size distribution function is computed automatically by a dedicated computer software. A kinetic analysis is performed for unstable dispersive systems.

MICROWAVE SAMPLE DIGESTION TECHNIQUE

The preparation of foodstuff and solid samples for assaying heavy metals is a laborious and time-consuming stage of the chemical analysis, which calls for much carefulness and persistent attention of an operator. It is during this stage when the largest errors are commonly brought about in the results of an experiment.


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