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HPLC System

APPLICATION NOTES Types of analysis Download PRESENTATION: HPLC with FLUORAT-02 detectors   

METHOD

High performance liquid chromatography 

PRINCIPLE OF OPERATION

Is based on the high-pressure syringe pump with fluorometric/spectrofluorometric/
photometric/spectrophotometric detectors
, premium quality columns.

FEATURES AND BENEFITS

  • Flexible modular system configuration allows building systems to meet the needs for the majority of the HPLC application
  • Simultaneous use of two detectors and compatibility with all types of HPLC-detectors makes analysis even more informative
  • Thermostating columns are available
Liquid chromatograph LUMACHROM®

APPLICATION EXAMPLES

Environmental: identification of a source of pollution of water by petroleum hydrocarbons, PAH monitoring in various media, including benzo(a)pyrene.
Food industry and veterinary: determination of aflatoxins, vitamins, N-nitrosamines, benzo(a)pyrene in foodstuff.
Hematology: determination of amino acid structure of blood serum.

(MORE INFO>>)

 
SPECIFICATIONS
Semi-microcolumn HPLC
Pump type syringe chamber volume 35 ml
operating pressure up to 50 (30)* MPa
flow rate 1 - 5000* µl/min 
Injector Rheodyne®  sample volume 10 µl (1, 2, 5 µl)*
Column  inside diameter 2.1 mm (4, 6 mm)*
length 80, 100, 120, 150, 200 mm
sorbent Kromasil® (Ń18 and SIL)
particle size 5 µm
Pre-column  inside diameter 2.1 mm
length 10 mm
sorbent Kromasil® (Ń18 and SIL)
particle size 5 µm
Detectors “Fluorat® - 02-2M” Fluorometric detector detection limit for benzo(a)pyrene 0.5 pg
"Fluorat® - 02- Panorama" - time-programmable spectrofluorometric detector  The same detection limit.
Make it possible to monitor each of peaks in the optimum spectral conditions.
photometric detector wave-length 254 nm
spectrophotometric detector wave-length spectral range 190-360 nm
Flowsell   illuminated volume 1 µl
Data processing Chrom@Spec® software package,
PeakExpert® software package
Ampersand® Ltd.
Lumex® Ltd.
* - special request

 

EQUIPMENT AND OPTIONS
  • Base unit
  • fluorometric detector Fluorat®-02-2M, spectrofluorometric detector Fluorat®-02-Panorama, photometric detector
  • PC with Pentium® II or higher, Windows®-98 or higher versions
  • Chrom@Spec® software package for fluorometric and photometric detectors; PeakExpert® software package for spectrofluorometric detector
WARRANTY

All LUMEX equipment is covered by 12-months warranty

SERVICES

Upon request installation and commissioning of LUMEX instruments can be carried out on Customer's site by our service engineers. Consultations and initial training of attendance personnel with due account of Customer's specific needs are performed. Spare parts are delivered and repair is made upon Customer's request (free within the warranty period).


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