CAPEL-series Capillary Electrophoresis Systems

High performance capillary electrophoresis 

Based on the differential migration of componenys of aqueous samples within a narrow quartz capillary under the influence of the applied electric field. Separated solutes are quantitatively detected at the capillary outlet by high sensitive optical system based on direct or indirect UV absorbance.

• high separation efficiency
• low reagent consumption
• simple sample preparation
• compact benchtop design
• autosamplers (10 inlet and 10 outlet vials) in CAPEL-105 and CAPEL-105M
• user-friendly software
• implementation of various methods (capillary zone electrophoresis, micellar electrokinetic chromatography, capillary isoelectric focusing, capillary gel electrophoresis, capillary isotachophoresis

• full control of the instrument from the PC
• broadened range of pre-set temperatures for liquid cooling
• fully automated programmable system with different levels of task complexity - from manual operation through simple analysis to complex sequence of runs
• extended operation options such as spectra scanning and broader injection pressure range
• ability to apply controlled pressure simultaneously with the high voltage during analysis
• substantially modified capillary cassette to provide liquid cooling, simplify capillary replacement and increase the sensitivity

DESCRIPTION - Automatic pressure or electrokinetic sample injection - Built-in capillary rinsing system - Convenient access to the vials and capillary - Possibility of visual check of the position of the vials and capillary rinsing - Liquid cooling system in CAPEL103 and in CAPEL-105, CAPEL-105M - Built-in monochromator (190 - 380 nm) in CAPEL-105 and CAPEL-105M

Common features for all CAPEL instruments provide clear benefits for their users:

Spectrophotometric detector, placed on Capel-105M, enables an operator not only to select the most appropriate wavelength within the UV range (190 - 380 nm) but also to perform spectral scanning of separated compounds, thus supporting in peak identification.

Capel®-series CE systems can be supplied with validated measurement procedures (in accordance with EPA, ASTM and other standards).

• Soils (mobile forms of Co, Cu, Ni, Zn)
• Drinking, natural and waste water (Br -, I - and other inorganic and organic ions)

• Mineral and bottled water (inorganic cations and anions)
• Beverages and juices (inorganic cations and anions, sweeteners, antioxidants, vitamins, carbohydrates)
• Wines, brandies and vodkas (inorganic cations and anions, aromatic aldehydes, phenolcarbonyc acids, amino acids)
• Beer (inorganic cations and anions, hop and beer bitter acids (humulones and isohumulones, amino acids, vitamins)
• Tea, coffee (caffeine, catechin)
• Foodstuff (amino acids, synthetic dyes , organic acids, amines, proteins)

• Technological monitoring and patent medicines analysis
• Enantiomers separation

• Determination of inorganic cations and anions, amino acids and proteins in biological fluids
• Pharmacokinetics studies
• Protein separation

• Analysis of explosives and trace detection
• Drugs analysis

• Technological monitoring
• Composition determination of raw material and intermediate products

Detectors
CAPEL-103 with one wavelength UV-detector lets you detect at 254 nm. CAPEL-105M and CAPEL-105M is equipped with variable wavelength UV-detector, wavelength range 190 - 380 nm.

Vials
Standard 1.5 ml, minimal sample volume 100 µl.

Injection modes
Controlled pressure profile injection with variable peak pressure, programmable peak pressure range being 0 - 60 mbar
Programmable injection time

Electrophoresis power
Voltage range settable from 1 to 25 kV
(manually reversible polarity)
Current from 0 to 200 mA

System control
Membrane keyboard
4 string 20 characters and 30 string 16 characters LCDs in Capel-103 and Capel-105/105M, respectively
Data acquisition and processing system based on a standard PC and Chrom&Spec® software with MSWindows® user interface.

Chrom&Spec® software features
Real time electrophoregram visualization
Electrophoregram data processing
Computation of electrophoresis system parameters
Customized report output (hard copy and file), data exchanges with worksheets, databases and word processors
Extensive data processing algorithm customization
GLP and GALP compliance.
Data exchanges with worksheets, databases and word processors.

APPLICATION NOTES (more info) >>

• capillary electrophoresis system CAPEL®
  (103R, 103RT , 104, 104M, 105, or 105M model)
• spare capillary cassette
• software package (for 103R, 103RT , 104, and 105 models - Chrom@Spec, for 104M and 105M models - Elforun®)
• kits for analysis [on request] ; the CE-kits of any manufacturers are compatible with Capel®-series CE systems

All LUMEX equipment is covered by a 12-months warranty

Upon request installation and commissioning of CAPEL CE systems can be carried out on Customer's site by our service engineers. Consultations and initial training of attendance personnel with due account of Customer's specific needs are performed. Spare parts are delivered and repair is made upon Customer's request (free within the warranty period).